P-13: Histomorphometric Changes of The Skin in Rats Following Administration of Testosterone and Nettle

Testosterone is one of the important androgen. It has been reported that testosterone can have effects on hair follicles, dermal fibroblasts and interfollicular keratinocytes in vitro conditions. The purpose of this study is to investigate the effects of testosterone and nettle on skin structures and sebaceous glands. Materials and Methods: For this study, 20 healthy adult male wistar rats with average weight 250 to 300 g and approximately 3.5 - 4 months age were used. They were fed with standard diet. The rats were divided to four groups (5 rats in each group): the control group which the rat without injection, the testosterone group which the rats injected by testosterone with 10 mg/kg/ day dose, nettle group which the rats injected by nettle extract with 50 mg/kg/day dose. The almond oils group with 10 mg / kg / day dose. All injections were performed subcutaneously. The rats were easy drawing with chloroform. After 42 days, samples were taken from head skin and the 5-6μ thickness sections were made by paraffin embedding method and were stained by H&E and Masson trichrome.The histomorphometrical studies were done using Olympus BX51 microscope and Olysia software. Results: The results showed that the sebaceous glands increased significantly grew in the testosterone group than control group while histological changes were not observed between the control Group and nettles. Micrometrical results showed that thick of epidermis and dermis was increased in the testosterone group compared to other groups. Histological results showed that there are not difference in fibroblasts and fibers in the between group. Conclusion: The sebaceous glands increased significantly grew in the testosterone group than control group while histological changes were not observed between the control Group and nettles.